Specifications
- Innovative platform for cell adhesion/cell mechanics research or for other long range force experiments integrated into inverted research microscopes
- Compatible with inverted microscopes for combined experiments, e.g., Zeiss Axio Observer, Axiovert A1, Nikon Ti/TE 2000, Nikon Ti2, Olympus IX series and Leica DMi series
- Integrates with advanced optical imaging such as DIC, phase contrast, fluorescence, confocal microscopy, Superresolution.
- Compatible with upright macroscopes and stereo microscopes such as Zeiss AxioZoom V.16, Leica Z16 ApoA, Leica M205FA and Olympus MVX10
- Living cell studies in native environment with tempeature control, perfusion and gas flow (CO2) on coverslips with the JPK BioCell™ or 35mm Petri dishes with or without glass bottom from Wilco, BD, WPI with the JPK PetriDishHeater™
- All parts which came in contact with the sample are easy to clean
- Compatible with standard incubators (specify model)
- High-throughput determination of cellular interaction parameters through JPK software batch processing
- Single-molecule events, work of removal, maximum adhesion forces, number of unbinding events and viscoelastic parameters such as Young’s modulus in one system
- 100 μm additional z-range with capacitive sensors for sub-nm precision
- 100 μm focus tracking optional by PIFOC® system keeps the plane of interest in focus during force measurements (substrate or cell surface)
- Proven NanoWizard® AFM technology for high resolution imaging and force measurements
DOWNLOAD BROCHURELiterature
- Strong adhesion by regulatory T cells induces dendritic cell cytoskeletal polarization and contactdependent lethargy. Chen et al., Journal of Experimental Medicine 214 (2), 327-338 (2017)
- Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. Theodosiou et al., eLife 2016;5:e10130 (2016)
- Decoding Cytoskeleton-Anchored and Non-Anchored Receptors from Single-Cell Adhesion Force Data. Sariisik et al., Biophys. J. 109 (7), 1330–1333 (2015)
- Atomic Force Microscopy Reveals a Role for Endothelial Cell ICAM-1 Expression in Bladder Cancer Cell Adherence. Laurent et al., PLoS ONE 9(5): e98034 (2014)
- Covalent and Density-Controlled Surface Immobilization of E-Cadherin for Adhesion Force Spectroscopy. Fichtner et al., PLoS ONE 9(3): e93123 (2014)
- The Properties of Chondrocyte Membrane Reservoirs and Their Role in Impact-Induced Cell Death. Moo et al., Biophys. J. 105 (7), 1590–1600 (2013)
- A new quantitative experimental approach to investigate single cell adhesion on multifunctional substrates. Canale et al., Biosensors and Bioelectronics 48, 172-179 (2013)
- Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy. Friedrichs et al., Nature Protocols 5(7), 1353-1361 (2010)